phase 103 contrast microscope Search Results


cd11c  (Miltenyi Biotec)
96
Miltenyi Biotec cd11c
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Cd11c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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analytical ion microscope smi 300  (CAMECA Inc)
90
CAMECA Inc analytical ion microscope smi 300
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Analytical Ion Microscope Smi 300, supplied by CAMECA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phase+103+contrast+microscope/pm02911003-36-7-11?v=CAMECA+Inc
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analytical ion microscope smi 300 - by Bioz Stars, 2026-07
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nhbo 103 mercury vapor lamp  (Carl Zeiss)
90
Carl Zeiss nhbo 103 mercury vapor lamp
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Nhbo 103 Mercury Vapor Lamp, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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c2 confocal microscope  (Nikon)
99
Nikon c2 confocal microscope
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
C2 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phase+103+contrast+microscope/pm37333770-93-18-21?v=Nikon
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axio imager z2 microscope  (Carl Zeiss)
90
Carl Zeiss axio imager z2 microscope
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Axio Imager Z2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
axio imager z2 microscope - by Bioz Stars, 2026-07
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43 objective  (Carl Zeiss)
90
Carl Zeiss 43 objective
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
43 Objective, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yokogawa csu x1 scan head 103  (Nikon)
99
Nikon yokogawa csu x1 scan head 103
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Yokogawa Csu X1 Scan Head 103, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phase+103+contrast+microscope/pmc08157923__pnas__2021814118__sapp-43-44-63?v=Nikon
Average 99 stars, based on 1 article reviews
yokogawa csu x1 scan head 103 - by Bioz Stars, 2026-07
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fitc  (Rockland Immunochemicals)
93
Rockland Immunochemicals fitc
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Fitc, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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103 objective on a zeiss 700 confocal microscope  (Carl Zeiss)
90
Carl Zeiss 103 objective on a zeiss 700 confocal microscope
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
103 Objective On A Zeiss 700 Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phase+103+contrast+microscope/pm35990742-198-22-22?v=Carl+Zeiss
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103 objective on a zeiss 700 confocal microscope - by Bioz Stars, 2026-07
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1x pbs  (Thermo Fisher)
99
Thermo Fisher 1x pbs
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
1x Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axioskop microscope  (Carl Zeiss)
96
Carl Zeiss axioskop microscope
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Axioskop Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axioskop microscope - by Bioz Stars, 2026-07
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biostation im  (Nikon)
96
Nikon biostation im
Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of <t>CD11c+</t> DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).
Biostation Im, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of CD11c+ DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).

Journal: European journal of immunology

Article Title: Neutrophil extracellular traps exacerbate Th1-mediated autoimmune responses in rheumatoid arthritis by promoting DC maturation

doi: 10.1002/eji.201646542

Figure Lengend Snippet: Cl-amidine treatment attenuates the expression of MHC-II and CD86 costimulatory molecules on DCs derived from draining lymph nodes (dLNs). dLNCs from bCII-injected DMSO and bCII-injected Cl-amidine treated mice were collected 12 d post collagen injection and analyzed by flow cytometry. (A) Relative numbers of CD4+ T cells/5 × 105 total LNCs (n=18–21/group) and the percentage of CD4+ IFN-γ+ and CD4+IL-17+ cells (n=14–17/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, **p=0.0031, *p=0.04; ns=non significant). (B) Representative flow cytometry analysis and relative numbers of CD11c+ DCs/5 × 105 total LNCs are shown. Numbers in FACS plot denote frequency. Representative FACS histograms depicting the geometric mean fluorescence intensity (MFI) of MHC-II and CD86 expression between the two groups, are shown, as examined by flow cytometry. Data shown are from one experiment representative of three independent experiments with 2–3 mice/group per experiment. Relative numbers of CD11c+ cells/5 × 105 total dLNCs (n=19–21/group) between the two groups are shown. Results are expressed as mean ± SEM. Data are combined from four independent experiments with 4–5 mice/group per experiment. (Unpaired t-test, ns=non significant). Dot plots represent the MFI of dLNCs of each CIA-DMSO or CIA-Cl-amidine administered mouse (n=8–9) normalized to the average MFI of CIA group (MFI fold change). Mean is depicted. Data are combined from three independent experiments with 2–3 mice/group per experiment. (Unpaired t-test, ****p<0.0001).

Article Snippet: Reagents Fluorescent-conjugated monoclonal antibodies to CD80 (16-10A1), CD86 (GL-1), CD11c (N418), CD4 (RM4-5), CD25 (pc61), CD44 (IM7) were all from Biolegend, Ly6G (1A8) and IFN-γ (XMG1.2) from BD Pharmingen,, MHC class II (M5/114.15.2) from Miltenyi, IL-17 (eBio17B7) and brefeldin A from eBioscience.

Techniques: Expressing, Derivative Assay, Injection, Flow Cytometry, Fluorescence

CIA BM-derived NETs increase the inflammatory properties of myeloid dendritic cells. Bone marrow-derived dendritic cells (BMDCs) from naïve mice were treated with condensed supernatants containing NETs (CIA-NETs) and control supernatants (control). (A) Representative confocal microscopy image from CIA-NETs after costaining with DAPI and MPO. Original magnification; 63×, digital zoom; 3×. Scale bar; 3μm. Microscopy image is representative of five independent experiments. (B) Representative flow cytometry analysis of CD11c+ DCs. Numbers in FACS plot denote frequency. Representative FACS histograms are shown with the corresponding MFI of CD80 and CD86 expression levels on CD11c+ cells. Data shown are from one experiment representative of four independent experiments. Dot plots represent expression levels of CD80 and CD86 of BMDCs treated with CIA-NETs or control (n=9–12) normalized against the expression of untreated BMDCs (fold induction). Results are expressed as mean; data are combined from four independent experiments. (Unpaired t-test, **p=0.013, *p=0.04). (C) IL-6 secretion in culture supernatants of BMDCs treated with CIA-NETs (n=12) or control (n=10) expressed as fold increase based on the secretion of the untreated BMDCs. Results are expressed as mean ± SEM; data are combined from four independent experiments. (Unpaired t-test, *p=0.038).

Journal: European journal of immunology

Article Title: Neutrophil extracellular traps exacerbate Th1-mediated autoimmune responses in rheumatoid arthritis by promoting DC maturation

doi: 10.1002/eji.201646542

Figure Lengend Snippet: CIA BM-derived NETs increase the inflammatory properties of myeloid dendritic cells. Bone marrow-derived dendritic cells (BMDCs) from naïve mice were treated with condensed supernatants containing NETs (CIA-NETs) and control supernatants (control). (A) Representative confocal microscopy image from CIA-NETs after costaining with DAPI and MPO. Original magnification; 63×, digital zoom; 3×. Scale bar; 3μm. Microscopy image is representative of five independent experiments. (B) Representative flow cytometry analysis of CD11c+ DCs. Numbers in FACS plot denote frequency. Representative FACS histograms are shown with the corresponding MFI of CD80 and CD86 expression levels on CD11c+ cells. Data shown are from one experiment representative of four independent experiments. Dot plots represent expression levels of CD80 and CD86 of BMDCs treated with CIA-NETs or control (n=9–12) normalized against the expression of untreated BMDCs (fold induction). Results are expressed as mean; data are combined from four independent experiments. (Unpaired t-test, **p=0.013, *p=0.04). (C) IL-6 secretion in culture supernatants of BMDCs treated with CIA-NETs (n=12) or control (n=10) expressed as fold increase based on the secretion of the untreated BMDCs. Results are expressed as mean ± SEM; data are combined from four independent experiments. (Unpaired t-test, *p=0.038).

Article Snippet: Reagents Fluorescent-conjugated monoclonal antibodies to CD80 (16-10A1), CD86 (GL-1), CD11c (N418), CD4 (RM4-5), CD25 (pc61), CD44 (IM7) were all from Biolegend, Ly6G (1A8) and IFN-γ (XMG1.2) from BD Pharmingen,, MHC class II (M5/114.15.2) from Miltenyi, IL-17 (eBio17B7) and brefeldin A from eBioscience.

Techniques: Derivative Assay, Control, Confocal Microscopy, Microscopy, Flow Cytometry, Expressing

CIA-NETs treated BMDCs augments antigen (Ag)-specific Th1 responses in vitro. (A) Outline of the coculture experimental setup. BM from C57BL/6 (B6) mice was differentiated into BMDCs (CD11c+) in the presence of GM-CSF. At day 9 the immature BMDCs were pulsed with ovalbumin (OVA) protein in the presence or absence of CIA-NETs or control supernatants for 18hrs and subsequently cocultured with LNCs from OT-II transgenic mice. 48hrs later IFN-γ and IL-17 production was assessed by intracellular staining. (B) Gating strategy of CD4+ T cells and CD4+IFN-γ+ cells are shown. Numbers on the gates denote frequencies. Gates were set as indicated. Percentages of CD4+ IFN-γ+ and CD4+ IL-17+ cells in total LNCs derived from OT-II mice pulsed with OVA and treated with CIA-NETs (n=4) or control (n=6), are depicted. Results are expressed as mean ± SEM; data are combined from two independent experiments. (Unpaired t-test, *p=0.02).

Journal: European journal of immunology

Article Title: Neutrophil extracellular traps exacerbate Th1-mediated autoimmune responses in rheumatoid arthritis by promoting DC maturation

doi: 10.1002/eji.201646542

Figure Lengend Snippet: CIA-NETs treated BMDCs augments antigen (Ag)-specific Th1 responses in vitro. (A) Outline of the coculture experimental setup. BM from C57BL/6 (B6) mice was differentiated into BMDCs (CD11c+) in the presence of GM-CSF. At day 9 the immature BMDCs were pulsed with ovalbumin (OVA) protein in the presence or absence of CIA-NETs or control supernatants for 18hrs and subsequently cocultured with LNCs from OT-II transgenic mice. 48hrs later IFN-γ and IL-17 production was assessed by intracellular staining. (B) Gating strategy of CD4+ T cells and CD4+IFN-γ+ cells are shown. Numbers on the gates denote frequencies. Gates were set as indicated. Percentages of CD4+ IFN-γ+ and CD4+ IL-17+ cells in total LNCs derived from OT-II mice pulsed with OVA and treated with CIA-NETs (n=4) or control (n=6), are depicted. Results are expressed as mean ± SEM; data are combined from two independent experiments. (Unpaired t-test, *p=0.02).

Article Snippet: Reagents Fluorescent-conjugated monoclonal antibodies to CD80 (16-10A1), CD86 (GL-1), CD11c (N418), CD4 (RM4-5), CD25 (pc61), CD44 (IM7) were all from Biolegend, Ly6G (1A8) and IFN-γ (XMG1.2) from BD Pharmingen,, MHC class II (M5/114.15.2) from Miltenyi, IL-17 (eBio17B7) and brefeldin A from eBioscience.

Techniques: In Vitro, Control, Transgenic Assay, Staining, Derivative Assay